但是有时候我们在不能确认所挑克隆是否正确前我们还是需要保存平板。为了避免琼脂平板被微生物污染，将平板密封好是非常重要的。最长用的方法是1）用保鲜膜，2）用封口膜。如果你不能很好的使用封口膜，你会在后续的试验中用掉更多。该视频中，Dan将向您演示如何使用一小条封口膜就能很好地密封琼脂平板。记住，只需要一小条，在绕平板密封过程中要均匀拉伸封口膜。How to Use Parafilm with Agar PlatesSometimes when using an agar plate, you pick the colonies you need, and then throw the plate away. But most of the time, you will store the plate for a while until you are certain your cloning worked. To prevent any unwanted microbes from growing on your plate, it is important to seal the edges. The two most common ways of sealing plates are 1) using plastic wrap, or 2) using Parafilm.If you’ve never used Parafilm to seal an agar plate, chances are that you’ll use too much of it. Here Dan shows how just a small strip of Parafilm is enough, and how you don’t literally “wrap” the entire plate.So remember, just a small strip is all you need, and stretch it all the way around the plate!
但是有时候我们在不能确认所挑克隆是否正确前我们还是需要保存平板。为了避免琼脂平板被微生物污染，将平板密封好是非常重要的。最长用的方法是1）用保鲜膜，2）用封口膜。如果你不能很好的使用封口膜，你会在后续的试验中用掉更多。该视频中，Dan将向您演示如何使用一小条封口膜就能很好地密封琼脂平板。记住，只需要一小条，在绕平板密封过程中要均匀拉伸封口膜。How to Use Parafilm with Agar PlatesSometimes when using an agar plate, you pick the colonies you need, and then throw the plate away. But most of the time, you will store the plate for a while until you are certain your cloning worked. To prevent any unwanted microbes from growing on your plate, it is important to seal the edges. The two most common ways of sealing plates are 1) using plastic wrap, or 2) using Parafilm.If you’ve never used Parafilm to seal an agar plate, chances are that you’ll use too much of it. Here Dan shows how just a small strip of Parafilm is enough, and how you don’t literally “wrap” the entire plate.So remember, just a small strip is all you need, and stretch it all the way around the plate!
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